Journal: Science Advances
Article Title: Integrin inhibition facilitates fibrocartilaginous transformation in connective tissue in osteoarthritis
doi: 10.1126/sciadv.ady4112
Figure Lengend Snippet: ( A ) Dot plot shows notably increased ECM-related signaling pathways of FB4 ( COL5A1 + ) in ADD TMJ-OA RCT compared to normal RCT. ( B ) GO analysis shows potentially up-regulated gene functions of FB4 ( COL5A1 + ) in ADD TMJ-OA RCT compared to normal RCT. Top 15 GO terms of the enrichment factors are showed. MHC, major histocompatibility complex; ER, endoplasmic reticulum. ( C ) Dot plot shows the expression of integrin-related genes in FB4 ( COL5A1 + ). The red (blue) dots are the up-regulated (down-regulated) genes in ADD TMJ-OA RCT compared to normal RCT. Horizontal and vertical coordinates indicate the specificity of the α and β integrin subunit genes on FB4 ( COL5A1 + ) cells. ( D ) The chord diagram shows the ligand-receptor pairs of periostin signaling (left). Proportions of periostin signaling interaction strength are compared between normal RCT and ADD TMJ-OA RCT (right). ( E ) Left plot shows coexpression of ITGAV and ITGB5 in FB4 ( COL5A1 + ) cells on the 10x Visium capture slides. Proportions of the coexpression of ITGAV and ITGB5 in FB4 ( COL5A1 + ) cells are compared between normal RCT and ADD TMJ-OA RCT. ( F ) Immunofluorescent staining for integrin α V β 5 in human TMJ disk tissues. Red: integrin α V β 5 ; blue: DAPI. White dotted lines: boundary of the disk tissues. N = 3 independent samples from different donors. Scale bars, 500 μm (left) and 50 μm (right). ( G ) Semiquantification of the percentage of integrin α V β 5 + area. Data are presented as mean ± SD. N = 3 independent animals. The two-tailed t test is used for data analysis. ** P < 0.01.
Article Snippet: Primary antibodies, which included antibodies against integrin α 5 β V (MAB2528, AB_2280706, R&D Systems, USA) diluted to 1:100 in blocking solution, were incubated at 4°C overnight.
Techniques: Protein-Protein interactions, Immunopeptidomics, Expressing, Staining, Two Tailed Test
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